Anthocyanin Biosynthesis in Flowers of Matthiola incana Flavanone 3- and Flavonoid 3'-Hydroxylases
نویسندگان
چکیده
Anthocyanins, Flavonoids, Biosynthesis, Flavanon 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Matthiola incana Enzyme preparations from flowers of defined genotypes o f Matthiola incana contain two dif ferent hydroxylases for hydroxylation of naringenin in the 3and 3'-position, respectively. The 3-hydroxylase is a soluble enzyme and requires as cofactors 2-oxoglutarate, Fe2+ and ascorbate. Besides naringenin eriodictyol is a substrate for the 3-hydroxylase. The 3'-hydroxylase is localized in the microsomal fraction and requires NADPH as cofactor. Naringenin and dihydrokaempferol but not 4-coumarate or 4-coumaroyl-CoA are substrates for this enzyme. 3'-Hydroxylase activity is present only in genetic lines of M. incana with the wild-type allele b+.
منابع مشابه
Genetic Control of Flavanone 3-Hydroxylase Activity and Flavonoid 3'-Hydroxylase Activity in Antirrhinum majus (Snapdragon)
Anthocyanin Biosynthesis, Antirrhinum majus, Flavonoids, Flavanone 3-Hydroxylase, Flavonoid 3'-Hydroxylase, Genetic Control In flower extracts of defined genotypes of Antirrhinum majus, two different hydroxylases were found catalysing the hydroxylation of naringenin and eriodictyol in the 3-position and of naringenin in the 3'-position. The 3-hydroxylase is a soluble enzyme and belongs accordin...
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